Similarly, Gramolini et al. performed gel-free shotgun sequencing of proteolytic digests of ventricular tissue extracts from phospholamban (PLN-R9C) mutant mice exhibiting dilated cardiomyopathy using high performance multidimensional liquid capillary-scale chromatography (HPLC) coupled to automated data-dependent tandem mass spectrometry (LTQ linear ion trap mass spectrometer) [5]. Here, PLN is linked to dilated cardiomyopathy.