After classification, we found that a large number of the selected candidate EBF targets are transcription factors - for example, NR2F2 (nuclear receptor subfamily 2, group F, member 2, also called COUP-TFII) [38], HOXD10 (homeobox D10, also called HOX4D) [39], NSCL-1 (neuronal stem cell leukemia, also called XHEN1 and NHLH1) [40], NeuroD [33], EN-2 (engrailed 2) [41], AML1 (acute myeloid leukemia, also called RUNX1) [42,43], and EMX1 [44]. The gene discussed is EN2; the disease is acute leukemia.