We first determined the extent to which PVT1 expression is regulated by glucose in the kidney, and because diabetic nephropathy is characterized by excessive accumulation of extracellular matrix (ECM) in the glomeruli, we also assessed the effect of glucose on mRNA and protein expression of specific ECM components in relation to PVT1 in mesangial cells. The gene discussed is PVT1; the disease is diabetic kidney disease.