The association of high Treg infiltration with poorer survival in all tumours was confirmed in a multivariate analysis including age, lymph node status, grade, size, ER, HER2 status, hormone therapy and chemotherapy (OR 1.62, 95% CI 1.02 to 2.55, P = 0.040) (Supplementary Table S1a in Additional file 1). The gene discussed is ESR1; the disease is neoplasm.