In a screening process of fecal specimens with a qPCR assay targeting the α-toxin encoding the plc gene of Cl. perfringens, 12 samples of IBS subjects were positive with the quantities ranging from 5.6 × 105 to 2.7 × 109 genomic equivalents per gram of feces (Figure 1B). This evidence concerns the gene HSPG2 and irritable bowel syndrome.