Although gene sets associated with G protein-coupled receptor (GPCR) were not definitively enriched (p = 0.07) in the cystic kidneys at E17.5 (two gene sets associated with GPCR were enriched at E14.5 in Pkd1-/- mutants compared to WT) as shown in Additional file 13, we identified 17 up-regulated individual genes associated with GPCR. The gene discussed is PKD1; the disease is Renal cyst.