The high proportion of quadruple mutant dhfr genotype in endemic area may be: 1) because of the characteristics of mutant parasites associated with higher gametocytogenesis [40-43] which favour transmission of mutant parasites compared to wild type dhfr, and 2) north-eastern states of India are highly endemic for malaria and international borders with neighbouring countries surround the region. This evidence concerns the gene DHFR and malaria.