In the present study, we used quantitative methylation-specific PCR (Q-MSP) to evaluate methylation levels of a panel of cancer-related genes in a cohort of clinically well-characterized NSCLC samples, including CALCA, CDH1, DAPK1, EVX2, PAX6, and RASSF1A, and further explore the association of promoter methylation of these genes with PIK3CA amplification. Here, PAX6 is linked to non-small cell lung carcinoma.