We propose that any evaluation of the link between Akt phosphorylation in tumour samples and the prediction or prognosis of disease should focus on the phosphorylation of Thr308 and/or at least one downstream Akt substrate, rather than Ser473 phosphorylation alone, in order to more reliably determine the activity of the kinase and thus better guide disease prognosis and treatment. The gene discussed is AKT1; the disease is neoplasm.