The observation that Akt Thr308 phosphorylation is the better indicator of activity was most obvious in group Ib tumours in which the phosphorylation of Akt on Thr308 and all three Akt substrates were increased, but the phosphorylation of Akt on Ser473 was decreased (Figures 3 and 4B). The gene discussed is AKT1; the disease is neoplasm.