In all three group Ib tumours, where Akt Thr308 phosphorylation was increased but Ser473 was decreased, the phosphorylation of all three Akt substrates was increased (Figures 3 and 4B), strongly suggesting that Thr308 phosphorylation correlates best with the activity of Akt towards downstream substrates. The gene discussed is AKT1; the disease is neoplasm.