IFNA1 and infection: To assess whether HIV-1 infection affects the level of tetherin expression in DCs, iDCs, mDC-LPS and mDC-IFNα were separately infected with WT NL(AD8) and ΔVpu, and the expression of tetherin and HIV-1 Gag in DCs at 2 h and 3-7 days post-infection were assessed by immunoblotting.