MYD88 and abscess: To determine whether viable abscess-associated neutrophils, macrophages, or microglia recovered from the infected CNS of MyD88 KO or WT mice displayed any differences in inflammatory mediator expression, we performed microbead array analysis on conditioned supernatants from each cell type following a 24 h incubation period in vitro without bacterial re-stimulation, in an attempt to capture cellular activation states that were ongoing in vivo.