Our results demonstrated a dose-dependent selective suppression of AP-1 activity by berberine which was accompanied by suppression of c-Fos and JunD expression and their reduced involvement in functional AP-1 complex in HPV positive cervical cancer cells irrespective of infecting HR-HPV types whereas JunB that also participated in an active AP-1 complex remained unaffected. The gene discussed is FOS; the disease is cervical cancer.