To understand the mechanism of berberine-induced growth inhibition and to examine whether berberine-induced inhibition of cervical cancer cells was associated with the induction of apoptosis, SiHa and HeLa cells were treated with berberine and berberine-induced apoptosis was assessed using Annexin V-PI staining of the treated cells that identify specifically the cells undergoing apoptotic cell death and start expressing phosphatidylserine on their cell surface. Here, ANXA5 is linked to cervical carcinoma.