PCSK1 and infection: At day 3 after infiltration with 6 × 107 cfu ml-1 of Pst the amount of bacteria in nec1 was reduced, whereas Parkland had accumulated ca. 6-fold higher amount of Pst making the difference in bacterial growth between wt and nec1 statistically highly significant (p = 0.01, Student's t-test) at this stage of infection (Figure 3A).