This induces an increase of cytokines and chemokines, mimicking the infection of P.aeruginosa, which is an important step in generating the clinical symptoms of CF, as reported in several studies and reviews [50,51] Figure 7A show that exposure of IB3-1 to heat-inactivated PAO1 causes an increase of IL-8 mRNA production, as elsewhere reported. Here, CXCL8 is linked to infection.