When either Q84 or R84 TRB3 full-length cDNAs were transfected in human HepG2 hepatoma cell lines, as compared with control HepG2 cells, insulin-induced Ser473-Akt phosphorylation was reduced by 22% in Q84- and, of note, by 45% in R84-transfected cells [14]. This evidence concerns the gene INS and hepatocellular carcinoma.