Indeed, among the filtered predicted targets of the upregulated miRNAs in the GK rats in particular rno-miR-335, are enriched for several key exocytotic proteins (Table 2), some of which were shown to have reduced expression in human T2D cases including Stxbp1 (Munc18-1) whose 3′UTR fragment we have shown to interact with miR-335. This evidence concerns the gene STXBP1 and type 2 diabetes mellitus.