To analyze the impact of a STAT3-decoy ODN on the subcellular localization of STAT3 in the colon carcinoma cell line SW 480, a combination of subcellular fractionation, oligonucleotide pull-down and immunofluorescence microscopy was used, in non-stimulated cells in most experiments in order to reproduce physiological conditions as much as possible. Here, STAT3 is linked to colon carcinoma.