As shown in Figure 1E, U87MG cells showed a time-dependent increase of apoptosis induced by nutlin-3a (from 3.3 to 27% at 24 and 96 hours, respectively) when compared to DMSO vehicle control, whereas no apoptosis was observed in T98G cells, suggesting resistance to MDM2 inhibition in glioblastoma cell lines with mutant p53. This evidence concerns the gene TP53 and glioblastoma.