In the present study, we performed two-way average-linkage hierarchical clustering with Euclidian distance for a 22-gene set that included the following genes: VEGFA, ARAF, BRAF, OPN (SPP1), MMP2, KRAS, NRAS, TGFβ1, AKT1, HRAS, TIMP1, EGF, RKIP (PBP), FGF2, EGFR, RAF1, CDKN2D, TP53, CDKN2A (p14ARF/p16INK4A), MMP9 and MKI67, in 10 BC samples vs. 5 controls. The gene discussed is EGF; the disease is breast cancer.