Factors that remained independently associated with CIN2+ diagnosis in the multivariate model (Table 3) were age <35 years when compared to ≥35 years (aPR  =  3.22 95%CI 1.23–8.39), current tobacco use (aPR  =  3.69 95%CI 1.54–8.78), nadir CD4 T-cell count <350 cells/mm3 when compared to ≥350 cells/mm3 (aPR  =  6.03 95%CI 1.50 – 24.3) and concomitant diagnosis of VIN and/or VaIN (aPR  =  2.68 95%CI 0.99–7.24). The gene discussed is CD4; the disease is vulvar intraepithelial neoplasia.