To further demonstrate the important role of SIRT3 in OSCC carcinogenesis, we used an in vivo murine floor-of-mouth model that mimicked human OSCC.23, 27 This model has the advantage that tumors injected into the floor of the mouth behave like human head and neck squamous cell carcinoma, growing in the same environment, growing aggressively, and invading surrounding tissues. Here, SIRT3 is linked to head and neck squamous cell carcinoma.