TWIST1 and neoplasm: These results obtained from Kaplan-Meier analyses at each tumor stage may seem an artefact, due to the low number of samples in a group, such as stage I. However, our conclusions are supported by the results obtained from another approach, i.e. LNM status or treatment protocols in OS, where the study was not affected by the number of samples and showed that in both cases TWIST1 mRNA expression levels have prognosis value only in early stages.