We found that the contractile ability of SSc fibroblast was reduced by 16% at the 24th hourly time point after TSP1 expression knockdown; in addition, TGFβ-induced contractility of both normal and SSc fibroblasts were diminished by 18% and 29%, respectively, at the 24-h time point. The gene discussed is TGFB1; the disease is systemic sclerosis.