To extend our data obtained by western blot analyses indicating that LSKL peptide reduced ERK activation and α-SMA expression in SSc fibroblasts, we employed indirect immunofluorescence analysis to show that a 24-h treatment of SSc fibroblasts with LSKL peptide reduced the appearance of α-SMA stress fibres and the intense p-ERK staining, both key features characterising SSc fibroblasts, Moreover, the LSKL peptide also blocked TGFβ-induced α-SMA expression and p-ERK activity in normal and SSc fibroblasts (Figure 2b). Here, TGFB1 is linked to systemic sclerosis.