Cut-off values also differ depending on the assay used to measure LH (5,13,14,15,16) In our clinic, LH is measured using ICMA, and LH ≥5 IU/L is considered to be diagnostic for CPP (13), whereas LH should be below 2 IU/L in order to consider a patient with CPP under GnRH analogue treatment as adequately suppressed (10). Here, PLOD1 is linked to central precocious puberty.