Moderately frequent methylation was found for MINT25, p16, RASSF1A and BLU (15.7, 15.7, 14.7 and 10.8%, respectively) and low frequency was observed for DAPK, CASP8, FAS, MGMT and p73 in 1–10% of CCA. Here, RASSF1 is linked to cholangiocarcinoma.