Moderately frequent methylation was found for MINT25, p16, RASSF1A and BLU (15.7, 15.7, 14.7 and 10.8%, respectively) and low frequency was observed for DAPK, CASP8, FAS, MGMT and p73 in 1–10% of CCA. The gene discussed is DAPK1; the disease is cholangiocarcinoma.