After excluding 14–3–3σ, 91% (93 out of 102) of CCA samples were methylated in at least one locus out of the 14 loci (OPCML, SFRP1, HIC1, PTEN, DcR1, MINT25, p16, RASSF1A, BLU, DAPK, CASP8, FAS, MGMT and p73). Here, DAPK1 is linked to cholangiocarcinoma.