The predicted RB-negative frequencies determined by the cut-off value of log10 (CCND1/CDKN2A) of 0.404 were 100% (7/7), 22% (2/9), 100% (5/5), and 5% (1/20) in cervical, endometrial, small cell lung, and gastric cancers, respectively (Table 2). The gene discussed is CCND1; the disease is gastric cancer.