CXCL8 and necrotizing enterocolitis: 1) The time needed to expose enterocytes to hydrocortisone (5 days) precluded using primary enterocytes particularly from NEC patients; 2) we have previously reported that hydrocortisone injection into SCID mice followed by an inflammatory stimuli (IL-1β) reduced the IL-8 response only in immature xenografts and qRT-PCR measurement of IκB mRNA from LCM of epithelial RNA one week after steroid treatment resulted in an increased IκB gene expression [9].