Therefore it is possible thatBmi-1 silencing leads to downregulation of Nrf-1 and or NFκB in ovarian cancercells, thereby decreasing transcription of GCLM resulting in reduced GSH synthesis.Importantly here we show that Bmi-1 by regulating ROS and GSH levels protects theovarian cancer cells from chemotherapeutic insults. This evidence concerns the gene NRF1 and cancer.