SIT1 and infection: To address whether the decreased survival of the sit1Δ strain could be attributed to altered levels of phagocytosis of the mutant yeast cells rather than decreased Fe satiety, CFU were monitored 2 hours post-infection and no significant differences between the wild type, sit1Δ and sit1Δ::SIT1 strains were observed in the presence or absence of ferrichrome, strongly suggesting equally efficient phagocytosis of the three yeast strains by macrophages (Figure S2A).