AFP and neoplasm: Moreover, immunohistochemical examination demonstrated that the tumor cells were positive for glypican-3 (GPC3) (Figure 5), AFP (Figure 6), hepatocyte paraffin-1 (Hep Par 1), cytokeratin (CK)18, and hepatocyte antigen, but negative for CK7, CK20, placental alkaline phosphatase (PLAP), prostate-specific antigen (PSA), cancer antigen 125 (CA125), epithelial membrane antigen (EMA), cluster of differentiation (CD) 117, and CEA, which confirmed that the seminal vesicle tumor was metastatic HCC.