LEF1 and colorectal carcinoma: This might suggest that Tcf-4 or Lef-1 have the ability to bind thepromoter region where site −110 is located if the conditions are satisfied.However if this is the site for interaction between Wnt signaling andNotch-2 in CRC is uncertain, and likely a larger part of thepromoter, and thereby the additional binding sites suggested by the insilico and EMSA analyzes, are needed.