Hes-1 has recently also been identified as a directtranscriptional target of β-catenin/Tcf-4-dependent Wnt signaling in CRC[31] andour EMSA data supports this notion by clearly identifying Hes-1-528 as a binding site for the β-catenin/Lef-1 complex (Fig.S1). Here, TCF4 is linked to colorectal carcinoma.