Pooled densitometric analysis of tumor-to-non-tumor ratios following IP experiments revealed a trend toward increased association of both AF-6 and PDZ-GEF2 with JAM-A, suggesting enhanced formation of a JAM-A/AF-6/PDZ-GEF2 signalling complex in tumor cells (Supplementary Figure S3 in Additional file 3). Here, RAPGEF6 is linked to neoplasm.