To independently verify that miRNA changes observed in CLL cells are characteristic of an activated B cell status, we purified B cells from healthy donors and stimulated these cells with a variety of B cell activators including anti-IgM and CD40L (BCR and T cell-assisted co-stimulatory pathways), and LPS, CpG, or polyI∶C (Toll-like receptor pathways) and examined miRNA expression. This evidence concerns the gene BCR and B-cell chronic lymphocytic leukemia.