To compare the iron content of CSF Tf between CJD- and CJD+ cases, first the iron saturation of CSF Tf from a relatively normal CJD- case was determined by competing radiolabeled 59FeCl3 with decreasing concentrations of unlabeled FeCl3 and fractionating 59Fe-Tf on a native gel for quantification (Figure S2 A, B). The gene discussed is TF; the disease is Creutzfeldt Jacob disease.