We also showed anti-tumor activity of CDF alone and in-combination with gemcitabine, which was consistent with inactivation of miR-21, and consequently increased expression of PTEN, attenuation of the DNA binding activity of NF-κB inhibition in the expression of COX-2, and activation in the expression of miR-200 in tumor remnants of a xenograft mouse model of human PC, all of which provide convincing in vivo activity of CDF which is consistent with in vitro findings. This evidence concerns the gene PTEN and neoplasm.