In the initial methylation profiling analysis using GoldenGate® Methylation Cancer Panel I array we included 17 samples from healthy donors (7 PBMNC, 5 CD19-positive cells from PBMNC and 5 CD3-positive cells from PBMNC), 48 ALL patient samples (17 BCR-ABL1+, 7 TEL-AML1+, 3 MLL+, 3 MYC+, 2 E2A-PBX1+, 4 common ALL, 1 pro-B ALL, 1 pre-B ALL and 10 T-cell ALL) and 4 ALL cell lines. The gene discussed is PBX1; the disease is acute lymphoblastic leukemia.