CALR and neoplasm: In order to determine if the observed phenomenon is also applicable to tumor-bearing mice, C57BL/6 mice (5 per group) were challenged with TC-1 tumor cells subcutaneously, vaccinated with pcDNA3-CRT/E7 DNA vaccine via gene gun delivery, and treated with DMXAA either before the first vaccination (d-3) or after the first vaccination (d+3) as indicated in Figure 4A.