To determine the role of VEGF induced tyrosine phosphorylation in H2461 mesothelioma cells that expressed PKC-β1 and PKC-β2, we pre-treated serum-starved mesothelioma H2461 and Met5A cells with or without the indicated concentrations of KRN633 (4 hours) and enzastaurin for 24 hours [Figure 3a]. The gene discussed is VEGFA; the disease is mesothelioma.