The 12 CpG loci identified by PAM analysis that provided the most accurate prediction of melanoma were RUNX3_P393_R, RUNX3_P247_F, RUNX3_E27_R, COL1A2_E299_F, MPO_P883_R, TNFSF8_E258_R, CD2_P68_F, EVI2A_P94_R, OSM_P188_F, ITK_P114_F, FRZB_P406_F, and ITK_E166_R. The gene discussed is ITK; the disease is melanoma.