In malignant melanoma, RT-PCR of CSF using primers for MART-1, MAGE-3 and tyrosinase (all known melanoma markers) were positive for at least one of the markers in 12 patients (vs. 1 positive cytology) of the 37 patients tested, and correlated significantly with development of CNS metastasis at 3 months [39]. This evidence concerns the gene MAGEA3 and melanoma.