We previously showed that MSP and sequence analysis of bisulfite-modified DNA forthe determination of the MGMT promoter methylation statusrevealed identical and reproducible results throughout the solid tumor space,even for small amounts of starting DNA as are obtained from a single1-mm3 stereotactic biopsy sample of a malignant glioma [20],[24]. The gene discussed is MGMT; the disease is malignant glioma.