To investigate a potential mechanistic link between p53 and DSC3 expression in colon cancer cells, we selected three cell lines including CX-2 (DSC3-unmethylated, p53-mutant), WiDr (DSC3-methylated, p53-mutant), and HRT-18 (DSC3-methylated, p53-wild type) for transfection with a wild-type p53 expression vector. This evidence concerns the gene DSC3 and colonic neoplasm.