To validate our computational analysis and better understand how WEE1 is regulated by miRs, we first assayed the absolute levels of WEE1 mRNA expression in 5 GA subgroup GBMs, 5 non-tumor brain samples, four GBM-derived tumor initiating/stem cell lines (TICs) and two normal cell lines (human fibroblasts and HUVEC), using quantitative reverse transcription polymerase assays (RT-qPCR). This evidence concerns the gene WEE1 and neoplasm.