Abolishment of the EHEC-Hly cytolytic activity towards the brain microvascular endothelium using recombinant strains coexpressing EspPα further demonstrates that EspPα may act as an inactivator for EHEC-Hly in cells that are affected during extra-intestinal complications of EHEC infection, such as HUS. The gene discussed is KRCC1; the disease is hemolytic-uremic syndrome.