In recent studies, PMCA was employed for generating infectious prions (PrPSc) in vitro de novo in crude brain homogenate [9], and for producing infections prions from the cellular prion protein (PrPC) purified from normal mammalian brains [10] and recombinant PrP (rPrP) produced in E. coli[11]. The gene discussed is PRNP; the disease is infection.