PRNP and scrapie: In order to determine the cause of our incapacity to detect abnormal PrP in the peripheral tissues of Atypical/Nor98 scrapie cases that contain infectivity, classical scrapie (cases 10 and 12) and Atypical/Nor98 scrapie (cases 1, 8, 9) brain homogenates dilution series were prepared and processed for a OIE registered PrPSc detection WB (TeSeE WB Kit – BIORAD), a PrPSc ELISA detection assay (TeSeE Sheep and Goat - BIORAD) (Figure 3 and Figure S1) and bioassays in tg338 mice (Table 3).