To determine the effect of EBNA3C and Cyclin D1/CDK6 complex on pRb mediated cell growth suppression, an osteosarcoma cell line, Saos2, was transfected with the expression plasmids for myc-tagged pRb, flag-tagged Cyclin D1, flag-tagged CDK6 and EBNA3C as indicated in the figure (Fig. 8A–D). Here, CCND1 is linked to osteosarcoma.