While in macrophages β-actin-Dendra2 fusion proteins showed similar distribution in the cell body irrespective of whether Dendra2 was fused to the N- or C-terminus of the β-actin cDNA, in carcinoma cells Dendra2 had to be fused to the C-terminus of β-actin so as to prevent its sequestration from the cytoplasm and its incorporation into stable stress fibers, which are not present in macrophages. Here, ACTB is linked to carcinoma.