The RQ values after calibrating with the Normal samples (Mean) for all the 94 genes showed 8 additional genes to be overexpressed; 4 (ASB16, CCL18, FST, THOC6) in Cancers, 1 (KLK9) in CIN3/CIS and 3 (RASSF6, TMEM123 and GLB1L3) in CIN1/2 samples. The gene discussed is RASSF6; the disease is in situ carcinoma.