PIK3C2A and myocardial infarction: As the pathological changes of myocardial injury caused by acute or multiple ISO treatment resemble the clinical manifestations of myocardial infarction [10,20,21], eg the ISO-induced necrotic cells' leakage of housekeeping enzymes such as LDH, AST and CPK from the myocardium to blood, the measurement of these enzyme activities is a reliable assessment for the extent of ISO-induced myocardial injury.